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A central message of the paper is both simple and often misunderstood: people smoke for nicotine, but most smoking-related disease comes from burning tobacco

concluded that the majority of users are attempting to quit smoking traditional tobacco cigarettes, while only 1% are new users or experimenters who did not previously smoke tobacco cigarettes

Pack-Year Meaning: Why Doctors Use 20 Cigarettes as a Pack The 20-cigarette standard is also used in health discussions

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First, linearized plasmid DNA (pIDTblue/EBOVL1-TMVshort) was used to optimize the reaction conditions for the qPCR assay (Supplementary Information, Fig